Available Tests and Fees

List of molecular detection assays for plant pathogenic bacteria, viruses, viroids and fungi that will be available at the Molecular Diagnostic Lab, UGA, Tifton. Molecular test per sample is $60.00, or otherwise specified below:

Bacteria | Viruses/Viroids | Fungi | Nematodes | Fungicide Resistance Screening

BACTERIA	DISEASE NAME	TEST DESCRIPTION	COST/SAMPLE	PANEL COST
Acidovorax citrulli	Bacterial fruit blotch of cucurbits	Pathogen isolation, DNA extraction, real-time PCR	$60
Candidatus Liberibacter asiaticus	Citrus greening (Huanglongbing or HLB)	*USDA APHIS standardized real-time PCR protocol	$80
Xanthomonas campestris pv. campestris	Black rot of cole crops	Pathogen isolation, DNA extraction, PCR, gel electrophoresis	$60
Xanthomonas campestris pv. raphani	Leaf spot of brassicas	Pathogen isolation, DNA extraction, PCR, gel electrophoresis	$60
Xanthomonas citri subsp. citri	Citrus canker	ImmunoStrip	$20
Xanthomonas citri subsp. citri	Citrus canker	DNA extraction and Real Time- PCR	$60
Xylella fastidiosa	Phony peach disease, Plum leaf scald, Pierce’s disease, Citrus variegated chlorosis, Leaf scorch in other hosts	DNA extraction, Real Time- PCR or isothermal PCR	$70

VIRUSES & VIROIDS	HOST	TEST DESCRIPTION	COST/SAMPLE	PANEL COST
Citrus leaf blotch virus (CLBV)	Citrus	RNA extraction, RT-PCR	$60	All three – $120
Citrus tristeza virus (CTV)	Citrus	ELISA	$40
Citrus Viroid II (Hop stunt viroid; HSVd)	Citrus	RNA extraction, RT-PCR or isothermal amplification	$70
Cucumber mosaic virus	Vegetables	ELISA	$40
Cucumber mosaic virus	Vegetables	RT-PCR	$60
Grapevine-redleaf-associated virus (GRLaV)	Viral diseases on grapes	RNA extraction, PCR	$60
Grape leaf roll viruses (GLRaV-1, -2, and -3)	Viral diseases on grapes	RNA extraction, PCR	$60	All three – $120
Tomato ringspot virus (ToRSV)	Different hosts	ImmunoStrip	$20
Tomato ringspot virus (ToRSV)	Different hosts	RNA extraction, RT-PCR	$60
Groundnut ringspot virus (GRSV)	Various hosts – primarily peanut	ELISA	$40
Groundnut ringspot virus (GRSV)	Various hosts – primarily peanut	RNA extraction, RT-PCR	$60
Potyvirus – Group test	Various hosts	ELISA	$40
Tomato yellow leaf curl virus (TYLCV)	Tomato	DNA extraction, PCR	$60
Tomato chlorosis virus (ToCV)	Tomato	RNA extraction, RT-PCR	$60	All three – $120
Tomato chlorotic spot virus (TCSV)	Various hosts – primarily peanut	RNA extraction, RT-PCR	$60
Tomato spotted wilt virus (TSWV)	Various hosts – primarily tomato, peanut, and tobacco	ELISA	$40

FUNGI	DISEASE NAME	TEST DESCRIPTION	COST/SAMPLE	PANEL COST
Alternaria spp.	Alternaria leaf spot	Pathogen isolation, DNA extraction, PCR, gel electrophoresis	$60 + $20 (if sequencing needed)
Botrytis cinerea	Botrytis fruit rot	Pathogen isolation, DNA extraction, PCR, gel electrophoresis	$60 + $20 (if sequencing needed)
Colletotrichum spp.	Anthracnose	Pathogen isolation, DNA extraction, PCR, gel electrophoresis	$60 + $20 (if sequencing needed)
Neopestalotiopsis	Neopestalotiopsis leaf spot	Pathogen isolation, DNA extraction, PCR, gel electrophoresis	$60 + $20 (if sequencing needed)

NEMATODES	DISEASE NAME	TEST DESCRIPTION	COST/SAMPLE	PANEL COST
Meloidogyne species	Root knot nematode	*Pathogen isolation, DNA extraction, PCR	$60 + $20 (if sequencing needed)
*Available soon. Please check back on May, 2023.

PATHOGEN	DISEASES	HOST	COST IN VITRO ASSAY	*COST MOLECULAR ASSAY
Alternaria spp.	Alternaria leaf spot	Fruits and vegetables	$200/5 isolates	$300/5 isolates
Botrytis cinerea	Botrytis fruit rot	Fruits	$200/5 isolates	$300/5 isolates
Colletotrichum gloeosporioides	Anthracnose	Fruits and vegetables	$200/5 isolates	$300/5 isolates
Fusarium oxysporum	Fusarium wilt	Solanaceous	$200/5 isolates	$300/5 isolates
Monilinia fructicola	Brown rot	Stone fruits	$200/5 isolates	$300/5 isolates
Pseudoperonospora Peronospora Plasmopara	Downy mildew	Fruit and vegetables	NA	**$300/5 isolates
Erysiphe necator (syn. Uncinula necator)	Powdery mildew	Grape	NA	*$300/5 isolates
In vitro assay: The 5 fungal isolates will be individually screen in vitro using the following: *Azoxystrobin (QoI) *Pyraclostrobin (QoI) Prothioconazole (DMI) Difenoconazole (DMI) Pydiflumetofen (SDHI) Penthiopyrad (SDHI) Benzovindiflupyr (SDHI) Fluopyram (SDHI)
*QoI resistance confirmation: Analysis of three amino acid substitutions (G143A, F129L, G137R) in the cytochrome b gene associated with QoI resistance will be perform upon request. This service includes DNA extraction, PCR, gel electrophoresis, PCR product cleanup, sequencing, analysis.
**Amino acid substitutions at position 1105 (G1105) in the cellulose synthase 3 (CesA3) gene are linked to CAA resistance in oomycetes. This service includes DNA extraction, PCR, gel electrophoresis, PCR product cleanup, sequencing, analysis.