List of molecular detection assays for plant pathogenic bacteria and fungi that are available at the Molecular Diagnostic Lab, UGA, Tifton. Please contact the Lab Director for specific requests. Molecular test per sample is $60.00, or otherwise specified below:
Bacteria | Fungi | Fungicide Resistance Screening
| BACTERIA | DISEASE NAME | TEST DESCRIPTION | COST/SAMPLE |
| Acidovorax citrulli | Bacterial fruit blotch of cucurbits | Pathogen isolation, DNA extraction, real-time PCR | $60 |
| Candidatus Liberibacter asiaticus | Citrus greening (Huanglongbing or HLB) | *USDA APHIS standardized real-time PCR protocol | $80 |
| Xanthomonas campestris pv. campestris | Black rot of cole crops | Pathogen isolation, DNA extraction, PCR, gel electrophoresis | $60 |
| Xanthomonas campestris pv. raphani | Leaf spot of brassicas | Pathogen isolation, DNA extraction, PCR, gel electrophoresis | $60 |
| Xanthomonas citri subsp. citri | Citrus canker | ImmunoStrip | $20 |
| Xanthomonas citri subsp. citri | Citrus canker | DNA extraction and Real Time- PCR | $60 |
| Xylella fastidiosa | Phony peach disease, Plum leaf scald, Pierce’s disease, Citrus variegated chlorosis, Leaf scorch in other hosts | DNA extraction, Real Time- PCR or isothermal PCR | $70 |
| FUNGI | DISEASE NAME | TEST DESCRIPTION | COST/SAMPLE |
| Alternaria spp. | Alternaria leaf spot | Pathogen isolation, DNA extraction, PCR, gel electrophoresis | $60
+ $20 (if sequencing needed) |
| Botrytis cinerea | Botrytis fruit rot | Pathogen isolation, DNA extraction, PCR, gel electrophoresis | $60
+ $20 (if sequencing needed) |
| Colletotrichum spp. | Anthracnose | Pathogen isolation, DNA extraction, PCR, gel electrophoresis | $60
+ $20 (if sequencing needed) |
| Neopestalotiopsis | Neopestalotiopsis leaf spot | Pathogen isolation, DNA extraction, PCR, gel electrophoresis | $60
+ $20 (if sequencing needed) |
| Other fungi | | Pathogen isolation, DNA extraction, PCR, gel electrophoresis | $60
+ $20 (if sequencing needed) |
| PATHOGEN | DISEASES | HOST | COST IN VITRO ASSAY | *COST MOLECULAR ASSAY |
| Alternaria spp. | Alternaria leaf spot | Fruits and vegetables | $200/5 isolates | $300/5 isolates |
| Botrytis cinerea | Botrytis fruit rot | Fruits | $200/5 isolates | $300/5 isolates |
| Colletotrichum | Anthracnose | Fruits and vegetables | $200/5 isolates | $300/5 isolates |
| Fusarium oxysporum | Fusarium wilt | Solanaceous | $200/5 isolates | $300/5 isolates |
| Monilinia fructicola | Brown rot | Stone fruits | $200/5 isolates | $300/5 isolates |
| Fusicladium effusum | Pecan scab | Pecan | $200/5 isolates | $300/5 isolates |
In vitro assay: The 5 fungal isolates will be individually screen in vitro using the following:
*Azoxystrobin (QoI)
*Pyraclostrobin (QoI)
Prothioconazole (DMI)
Difenoconazole (DMI)
Pydiflumetofen (SDHI)
Penthiopyrad (SDHI)
Benzovindiflupyr (SDHI)
Fluopyram (SDHI) |
| *QoI resistance confirmation: Analysis of three amino acid substitutions (G143A, F129L, G137R) in the cytochrome b gene associated with QoI resistance will be perform upon request. This service includes DNA extraction, PCR, gel electrophoresis, PCR product cleanup, sequencing, analysis. |